Normal human peripheral blood cells were either left unstained (blue histogram) or stained with CD54 (ICAM-1) Monoclonal Antibody, NovaFluor Blue 610-70S (purple histogram). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.